Susceptibilities of adults from newly established colonies of Culex salinarius from New Jersey and Texas to commonly used mosquito adulticides were assessed using the insecticide-coated vial bioassay technique. Females from both colonies were similar in their susceptibilities to naled, chlorpyrifos, resmethrin, and permethrin. However, females from the New Jersey colony (established from collections made in Cape May County, NJ) were found to be 9 times more tolerant to malathion than were those from the Texas colony (established from collections made in Chambers County, TX), with median lethal concentration values for malathion tested against these 2 colonies of 0.70 and 0.08 microgram malathion/vial, respectively. The differences between these 2 colonies with respect to their tolerances to malathion may be a product of the age of each colony at the time assessments were made and/or the degree to which the parent stock used to start each colony was previously exposed to malathion in the field.