Methods are described for the laboratory colonization of Aedes notoscriptus from Brisbane, Queensland, Australia. To initiate colonization, efforts were made to duplicate the natural environment of this species, including the use of a bromeliad as a swarm marker and oviposition substrate. The colony stabilized after the F8 with eclosion rates >50%, and an average production of 5,220 adults since the F9. The fecundity of Ae. notoscriptus averaged 29.4+/-10.5 eggs (range 14-57). The average development times from egg hatch to adult were 11 and 20 days under typical summer (20.5-28.9 degrees C) and winter (10.1-21.2 degrees C) conditions in Brisbane, respectively. This is the 1st published report of the colonization of Ae. notoscriptus.