Classifying individual mosquitoes of the Anopheles quadrimaculalas species complex to specieshas always been time consuming and complex, involving genetic analysis and profiling. We characterized thewingbeat frequencies of the 3 species of the Anopheles quadrimaculanrs complex (An. quadrimaculatus, An.smaragdinus, and An. maverlius) that occur in Mississippi to determine if this character could successfullydistinguish between individuals of the 3 species. Wingbeats of f'emales varied from 320 to 480 beats/sec, butno significant difl-erences in wingbeat frequencies were found among f'emales from 3 populations of the 3 species.Wingbeats of males were higher than those of females (500-770 beats/sec), and the mean wingbeat frequencyof An. maverlias was significantly difl'erent from the means of An. quadrimaculatus and An. smaragdinus,although overlap occurred at the individual level. Such overlap precludes use of wingbeat f'requencies as anidentification mechanism, and indicates that, at least for the Az. quadrimaculatas complex, wingbeat frequenciesare not involved in mate recognition.