Data on the biology and laboratory maintenance of Aedes trivittatus (Coq) are reported. Methods used to obtain large numbers of Ae. Trivittatus for laboratory experimentation with minimum effort involve the collection and storage of eggs from field-collected adults. Each field-collected female produces an average of 62 eggs and nearly 50% hatch, even after storage of up to 1 year. Eighty % survival of larvae and 97% survival of pupae are obtained by using the rearing methods described. Fifty % of adult females survive for more than 26 days, with maximum survival for any mosquito being 60 days. Problems and precautions involved in maintaining Ae. trivittatus in the laboratory are discussed.