Culex (Culex) restuans, Culex (Melanoconion) opisthopus and Culex (Melanoconion) erraticus were tested for susceptibility to Plasmodium hermani, a malaria parasite of wild turkeys (Meleagris gallopavo) in Florida. The sporogonous cycle was completed in Cx. (Cux.) restuans and mosquitoes with infected salivary glands transmitted the infection to domestic turkey poults by bites. Transmission was achieved also by intraperitoneal, intravenous and intra-muscular injections of whole body slurry. This is the third mosquito, belonging to the subgenus Culex, found to be susceptible to wild turkey malaria. Culex (Mel.) Opisthopus and Cx. (Mel.) erraticus did not become infected.