Aedes aegypti were collected and identified from boats arriving at Port-of-Spain, Trinidad, West Indies. A total of 46,895 boats was inspected, of which 31 had Ae. aegypti breeding in containers (0.07%). The containers which supported breeding aboard boats were water drums (54%), plastic containers (22%), water tanks (18%), lifeboats (4%) and tubs (2%). Boats coming from South America and the Caribbean may have been responsible for re-introducing Ae. aegypti into Trinidad. The need for more effective port health surveillance measures is recommended.