Anopheles (Anopheles) bradleyi King, An. (Ano.) crucians Wiedemann and An. (Ano.) georgianus King are taxonomically redefined by morphology, ethology and distribution, and established as the crucians subgroup of the An. (Ano.) punctipennis (Say) species group. This study involved the examination of over 1800 specimens and the preparation of 15 full-page illustrations. Species distributions include sections on: type-data, synonymy, descriptions of female, male, pupa, and larva distribution, taxonomic discussion, bionomics and medical importance. Keys for the crucians subgroup are presented for male genitalia, pupae and 4th stage larvae. Additional keys are presented, in an appendix, to separate the crucians subgroup from the other southeastern United States Anophelines.