i 
WHR Oy go 
Car. III.] THE PROCESS OF AGGREGATION. 41 
morning, after 22 hrs., by which time some new minute spheres had 
been formed; these oscillated from side to side and changed their 
positions, proving that the current had not ceased, though no stream of 
protoplasm was visible. On another occasion, however, a stream was 
seen flowing round the cell-walls of a vigorous, dark-coloured leaf, 
after it had been left for 24 hrs. in a rather stronger solution, namely, 
of one part of the carbonate to 218 of water. This leaf, therefore, was not 
much or at all injured by an immersion for this length of time in the 
above solution of two grains to the ounce; and, on being afterwards left 
for 24 hrs, in water, the aggregatcd masses in many of the cells were 
redissolved, in the same manner as occurs with leaves in a state of 
nature when they re-expand after having caught insects. 
In a leaf which had been left for 22 hrs. in a solution of one part of 
the carbonate to 292 of water, some spheres of protoplasm (formed by 
the self-division of a bag-like mass) were gently pressed beneath a 
covering glass, and then examined under a high power. They were 
now distinctly divided by well-defined radiating fissures, or were 
broken up into separate fragments with sharp edges, and they were 
solid to the centre. In the larger breoken spheres the central part was 
more opaque, darker-coloured, and less brittle than the exterior; the 
latter alone being in some cases penetrated by the fissures. In many 
of the spheres the liue of separation between the outer and inner parts 
was tolerably well defined. The outer parts were of exactly the same 
very pale purple tint, as that of the last-formed smaller spheres ; and 
these latter did not include any darker central core. 
From these several. facts we may conclude that, when vigorous dark- 
coloured leaves are subjected to the action of carbonate of ammonia, 
the fluid within the cells of the tentacles often aggregates exteriorly 
into coherent viscid matter, forming a kind of bag. Small spheres 
sometimes appear within this bag, and the whole generally soon divides 
into two or more spheres, which repeatedly coalesce and redivide. After 
a longer or shorter time the granules in the colourless layer of protoplasm, 
which flows round the walls, are drawn to and unite with the larger 
spheres, or form small independent spheres; these latter being of a 
much paler colour, and more brittle than the first aggregated masses. 
After the granules of protoplasm have been thus attracted, the layer 
of flowing protoplasm can no longer be distinguished, though a current 
of limpid fluid still flows round the walls. : 
If a leaf is immersed in a very strong, almost concentrated, solution 
of carbonate of ammonia, the glands are instantly blackened, and they 
secrete copiously; but no movement of the tentacles ensues, ‘lwo 
leaves thus treated became after 1 hr. flaccid, and seem killed; all the 
cells in their tentacles contained spheres of protoplasm, but these were 
small and discoloured. Two other leaves were placed in a solution not 
quite so strong, and there was well-marked aggregation in 30 m. After 
24 hrs. the spherical or more commonly oblong masses of protoplasm 
became opaque and granular, instead of being as usual translucent : 
and in the lower cells there were only innumerable minute spherical 
