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506 



MB. S. LE M. MOORE S STUDIES 





hereafter, when treating of Ballia calUtricha *. The only proteid 

 reaction yielded by the sieve-tube tissue was the xanthoproteic; 

 but I could never find a trace of reddening with Millon's reagent, 

 nor did copper sulphate and caustic potash or sugar and sulphuric 

 acid tinge the walls to the slightest extent. 



(b) Cellulose Beaction.— On the other hand, the walls of the 

 sieve-tubes and cambiform and companion cells give the purple 

 colour with Schulze's solution, by which cellulose is known. The 

 slime aud callus are coloured brown by this reagent. 

































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Action of a Peptonizing Fluid upon Vegetable - 



Maerow Callus. 



Marrow 



the chief reactions employed in the detection of proteids. Aud 

 here arises a question of some interest, namely, whether this 

 callus-proteid resembles the main body of proteids in being 

 acted on by pepsin and typsin in an acid and alkaline medium 

 respectively with formation of peptone ; or whether, like lardaceiu, 

 it is able to withstand the action of proteolytic ferments. In 

 order to answer this question, experiments were arranged in the 

 following way. 



Into the first of four vessels was placed some artificial gastric 

 juice prepared according to Pharmacopoeia directions, and some 

 radial or tangential sections of the phloem of the Vegetable- 

 Marrow. The second vessel contained some gastric juice with 

 finely-chopped meat; this was to serve as a test of the good- 

 ness of the pepsin. Sections of phloem with some 02 per ce 

 solution of hydrochloric acid were in the third vessel ; the object 

 of this was to ascertain whether the acid alone would have any 

 action upon the callus ; and, if any, what action. The fourth 

 vessel contained distilled water alone. All four vessels were 

 then exposed to temperatures varying between 35°-39° C. From 

 the second vessel the peptone reaction was obtained after a little 

 time, thus showing the genuineness of the pepsin. Sections from 

 the first vessel were examined from time to time ; and it soon 

 became evident that dissolution was in progress. So much was 

 this the case, that at the end of 8£ hours a considerable part ol 

 the callus had already disappeared j while after 16 hours no trace 

 of it remained upon the sieve-plates. The sieves were now 



cent. 



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