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514 



MR S. LE M. MOORE S STUDIES 

















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Reactions of the Cell-wall of Ballia. 



Xanthoproteic reaction. — This is given very fairly, but not 

 nearly so well as is the case with the protoplasm, and & fortiori 

 with the stoppers. No other proteid reaction is given by the 

 wall. 



Hydrochloric Acid neither alone nor after phloroglucin gives 

 a red colour to the wall. 



With Schulze's solution local blueing is caused, but the effect 

 is not general even after twenty-four hours. On the other hand, 

 boiling for a minute or two in caustic soda causes the whole 

 wall to take a deep blue-black colour. This points to the 

 presence of cutin in the cell-wall, and the action of Fuchsin 

 mentioned below confirms this idea. 



Sands s Picric Blue stains yellow the outer part of the wall of 

 large cells — the inner part taking a dark blue, and the whole, or 

 nearly the whole, of the intercellular septum is so stained. 



Watery JEosin stains the wall a brilliant permanent pink. 



Corallin-Soda also stains, but temporarily. 



Fuchsin colours the outer part of the wall brilliantly and 

 permanently. 



Strong Hydric Sulphate causes the inner layers to swell up 

 greatly. 



Hematoxylin stains the wall the characteristic bluish colour. 



"We may perhaps suspect from these reactions that there is 

 present in the cell-wall some substance or substances, either of 

 proteid nature or a decomposition product of proteids ; but the 

 entire failure of Millon's and the Copper Sulphate and Caustic 

 Potash tests, tells very much against this. 





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Some other Beactiofs of "Wall, Cell-Protoplasm, 



and Stoppers. 

 It is scarcely to be doubted that, as Archer* maintained, and as 

 I have myself also done my best to show t, there is no pit-closing 

 membrane in Ballia callitricha. This fact comes out clearly 

 when testing for Millon's reaction, the proteid contents of the 

 pit taking the brilliant colour of the stoppers and no break being 

 observable in the connecting column (Plate XIV. fig. 4). The 



* Trans. Linn. Soc. 2nd Series, i. 



t Journ. Linn. Soc., Bot. xxu, also Wright, Trans. B. Irish Acad. 1879 ; Hick, 

 Journ. of Bot 1884 ; Massee, Journ. R. Micros. Soc. 1884, &c. For the contrary 

 see Schmitz (Sitzungsb. d konigl. Akad. d. Wise, zu Berlin, 1883) and 

 iner (Proc. Camb. Phil. Soc. 1884. d. 104). 

















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