AS A FUNCTION OF LIVING PROTOPLASM. 293 
Lowest Min. |Highest Max.|| Lowest Min. |Highest Max.| Lowest Min. Highest Max. 
No. I. No. I. No. II. o. II. No. III. o. III, 
o o o o o o 
R...| 36 ... 010 | 52... 021 || 39... 020 |52... 021 |! 36... 033 |59 ... -035 
Y...[36 ...:029 | 56... 030 || 36.. 034*| 56... 030 ||38 ... 023 |47 ... 041t 
G...| 36 ... 020 | 52... 027 || 40... 022 | 52... 027 ||36 ... 036 | 56... 036 
V---{ 38... 016 | 47 ... 0271/40... 015 | 47... 027 || 36... 046 | 52... 042 
Cl...) 38 ... -030 | 53... 043 || 38... 031 | 53... 043 | 38... 063 | 53... -045 
These results are sufficient to show that temperature has a 
marked effect upon the transpiration, when no account is taken 
of any difference between light and darkness. The effect, 
however, becomes much more pronounced when Mushrooms are 
transferred from a warm room to a cool one, and allowed to 
remain in each for definite periods and of the same duration. 
Three Mushrooms were grown in separate pots and protected 
as before. They were placed for twenty-four hours alternately 
in a cool room with no fire in it, and in a warm one where a fire 
burned every day. They were always in total darkness, being 
enclosed in a cardboard box. The following are the total losses 
in twenty-four hours :— 
I II III Min. | Max 
1886 s grms. | grms o o 
Dec. 6- 7 Cool room...... 81:50 “82 116 50 56 
» "7-8 Warm ,, ..... 3:38 1:68 273 52 68 
” & 9 Cool „m ver 2:18 119 181 44 59 
» 9-10 Warm „m te 357 2:61 2:90 48 76 
» 10-1] Cool  , en 2°67 85 1:15 44 56 
» 11-12 Warm „ ...... 315 1:45 1:93 50 62 
» 12-13 Cool » eH :909 92 1:02 47 
» 13-14...| Warm , ...... 2:27 1:37 171 48 61 
Transpiration from Living Mushrooms compared with Evapora- 
tion from Saturated Dead Specimens. 
Two of the three specimens, viz. Nos. I. and II., together 
with No. IV., were scalded after the above experiments were 
* Exceptionally increased loss by dying and drying up rapidly. 
t Means of two observations. 
