OXYGEN FROM COLOURED BACTERIA. 139 
hours, could evolve per gramme of Bacteria 0:35 c.c. of oxygen. 
Similar cultures of Bacillus brunneus kept saturated with ether 
for two and four days could evolve per gramme of Bacteria 
after two days, in one case 0°45 c.c. of O, and in another 
03 c.c., and after four days, in one case 043 c.c. and in another 
0°24 c.c. 
By extraction with aleohol it is possible to obtain the pigment 
along with some otber extractives but free from all Bacteria, and 
to demonstrate that it still possesses the power of occluding and 
evolving oxygen. Thus if the alcoholic extract at (35° C.-40? C.) 
is allowed to evaporate to a small bulk and then water is added, 
a flocculent brownish precipitate is formed. The amount of 
precipitate thus obtained from 1 gramme of Bacteria was capable 
of evolving, after having been well shaken up with air, in one 
case 0322 c.c., in another 0:25 c.c., and in a third 0°11 c.c. of 
oxygen. 
A mass of B. brunneus was treated with alcohol at 40? C. till 
all Pigment was extracted and the residue was colourless. The 
residue weighed moist but with no superfluous water or alcohol 
2 grammes, and yielded 0-17 gramme of pigment and extractives. 
A fourth of this filtrate was, after precipitation with water, 
found to be capable of evolving per gramme of pigment 0:73 c.c. 
of oxygen. The remainder was allowed to evaporate to dryness 
and was weighed (weight —0:13 gramme), and was found to be 
capable of evolving in one case 0:23 c.c. of oxygen and in another 
021 cc. Hence in the process of preparation and drying the 
extracted pigment is apt apparently to lose its power of occluding 
ae and after keeping for a day or two the power is entirely 
the qued soia pigment thus extracted was also examined by 
compared with a} method, and its power of evolving oxygen 
of these with that of wood-charcoal and oxyhemoglobin. Both 
sufficient eee as the current of hydrogen has ceased, evolve 
in a few m M gen to set the Bacterium Termo in the hanging-drop 
Charcoal 0:9 es m active movement. A hemispherical piece o 
(capacity Lr On. diameter and 0:2 em. thick, in the gas-chamber 
oxygen in e to 1-4 c.c.), continues for several hours to evolve 
this pheno sa Pee amount. For the first 4 to 4 hour (though 
theless is veda not appear to be a vital one) oxygen never- 
continuous e ved so rapidly and in such quantity that even 1n à 
to more, After as ot pure hydrogen the B. Termo continue 
er being for 4 to 8 hours in hydrogen, however, 
