OXYGEN FROM COLOURED BACTERIA. 143 
100 c.c. Bouillon-culture +80 c.c. air and nearly 2 grms. 
Bacteria moist weight. 
O absorbed | OO, evolved | CO; 
Time. Amt. of O. | Amt. of CO,. per 3 hours. | per 3 hours. | O 
AM. P.M. 
930-1230 | 205 p.c. 0:66 p.c. 0:24 c.c. 0:528 ec. | 4s 
P.M. P.M. 
12.30-3.80 | 201 ,, 14 , 0:32 c.c. 0:672 c.c. | M 
3.30-6.30 | 186 ,, 19  , 123 ec. 04 cc. | $ 
630-930 | 177 ,, 22 p O72 c.c. 024 cc. | 1 
PM. A.M. 
9.30-9.30 1524 , 2475 ,, 0:402 c.c. 0:104 c.c. i 
100 e.c. of culture +60 c.c. air -- nearly 2 grms. of Bacteria. 
Time. Amt. of O. | Amt. of CO,. per 3 hours, Da howe. ny 
| 
A.M. P.M, 
10—1 20:5 p.c. 0776 p.c. 018 e.c. 0:456 ec. | 38 
P.M. P.M. 
m 200 ,, l5 , 030 ec. | 0444 cc. | i 
T IB3 , 198 ,, 1:02 cc. | 0288cc. | 3 
ved 169 , | 230 , | 078 cc. | 0192ce | d 
10—10 144 , 982 , 0324 c.e. | 0078cc. | 4 
Ina confined space, therefore, Bacillus brunneus at first respires 
at the expense of the oxygen held by the bacterial pigment, the 
; . ,C€0 
respiratory quotient -o being much greater than unity. After 
Sout 6 hours this oxygen has been apparently used up, and the 
Spiratory quotient is now much less than unity (3 to 1) and 
remains fairly constant. 
Another con 
which corrobo 
eld by a mas 
with 
clusion to which these results, along with others 
rate them, point, is that the amount of oxygen 
8 of coloured Bacteria and capable of providing it 
e o apply of respiratory oxygen is larger than that found by 
quantitative calculations previously given. 
amina tn of other coloured and uncoloured Bacteria were 
ed both dead and living, either by the Bacterium method 
or b . 
y the analytical method or both, but in no case could any 
