NUCLEI IN THE MTCETOZOA. 535 



1 p.m. Elaters pointed and showing faint spirals. 



7p.m. Elaters with well-forined spirals; nuclei unaltered; 

 many small deeply-stained nuclear bodies distributed through the 

 preparation (PI. XXXV. fig. 3). 



7.20, 8, 8.30, 9 p.m. No apparent change from 7 p.m. 



9.30 p.m. The spindle had formed with a thick nuclear plate 

 appearing as if composed of numerous short rods in confused 

 aggregation; spindle-fibres distinct (fig. 5). 



9.55. The two halves of the nuclear plate in different degrees 

 of separation. 



10.20. No spindles; a reticulum suggesting the "coil" stage, 

 — a less advanced development. 



10.40. The nuclear plate widely divided, a few retaining the 

 spindle-form (fig. 6). 



11.5. A remarkably beautiful and uniform preparation ; all 

 the nuclei of hexagonal figure, the two halves of the nuclear 



plate separated to the distance of their diameter ; the spindle- 

 fibres sharply defined. 



11.27. Daughter nuclei separated, discoid (fig. 7). 



11.47. No apparent difference from the last. 



Dec. 14, 12.5 a.m. An appearance of spore-formation beginning. 



12.20. A few spores seen of normal size. 



1.15. Spores increased in number. 



1.50. do. do. 



2.15. Some spores showing a delicate spore-wall (fig. S). 



A large growth of Trichia fragilis, which emerged from rotten 

 wood in October, gave sharp karyokinetic figures corresponding 

 W T ith those of T. fallax as far as stainings were taken, but they 

 were not carefully timed. 



Until quite recently I had not seen karyokinesis in Badhamia 

 utricularis. I was particularly anxious to observe it in this 

 species, because, as I have said before, I have cultivated the 

 Plasmodium for six years from one source and have made a large 

 number of stainings of the creeping stage with the object, if 



possible, of detecting the dividing nuclei. 



On Jan. 28 one of the cultivations, which had been fed 

 on Auricularia mesenterica for some months, showed signs of 

 changing to fruit, and at 10 o'clock at night it was seen to be 

 concentrating into sporangia. At 4 o'clock the next morning 

 between one and two thousand well-formed sporangia were 

 hanging in clusters, like golden grapes, from the fungus on which 

 the plasmodium had been feeding. From this time to 11.37 a.m. 



