CARBONATE OF AMMONIA ON CHLOROPHYLL-BODIES. 279 
globules of pale-blue transparent matter. In the preceding paper 
an analogous result from the action of carbonate of ammonia is 
described in the underground stems and rhizomes of Mercurialis 
perennis. The leaves were left for 24 additional hours in the 
solution, and now the horseshoe masses disappeared, being con- 
verted into pulpy matter. The immersion of the leaves of this 
Cyclamen in water for 47 hours caused the chlorophyll-grains to 
accumulate into heaps, as is known to follow from any injury 
there was hardly a trace of their confluence, and none of the 
pale-blue globules were present. Similar horseshoe masses were 
seen, but only on one occasion, in the leaves of Nicotiana taba- 
cum after their immersion in the solution; and so it was with 
the stems of Euphorbia Peplus. Portions cut from a leaf of 
Mirabilis Jalapa were left for 164 hours in solutions of 4 and of 
7 to 1000, and the chlorophyll-grains in many of the cells became 
completely confluent, forming horseshoe masses or rings; and 
they were sufficiently solid to project when the cells were torn 
open. When these horseshoe masses and rings were irrigated 
with acetic acid, they became so transparent that even their out- 
lines could hardly be distinguished. Ifin these plants, and more 
especially in Cyclamen and Mirabilis, the confluent chlorophyll- 
grains forming the horseshoe masses are still alive (and this is 
rendered probable by their bright-green colour, and in the former 
plant by their breaking up when left for an additional day in 
the solution, and in the latter plant by the action of acetie acid 
on them), we have in these cases a first step in the process which 
in some plants leads to the formation of spontaneously moving 
masses lying free in the cell-sap. ; 
Pelargonium zonale.—The effects produced by the immersion 
of the leaves of this plant for 24 or 48 hours in solutions of 4 or 
7 parts of carbonate of ammonia to 1000 of water are not a little 
perplexing. The leaves are clothed with glandular hairs, which 
absorb the ammonia and undergo aggregation. Moreover, nume- 
rous almost colourless, shining, translucent spheres generally, 
but not invariably, appear in most of the epidermal cells in 
which there are no grains of chlorophyll, and in the palisade- 
cells, in which they abound, and likewise in the parenchyma. 
The smaller spheres blend together, and thus form large ones. 
A solution of only 2 to 1000 sometimes sufficed to produce the 
spheres. Usually the spheres are not acted on by alcohol, but 
occasionally they were dissolved by it. If after immersion in 
