CARBONATE OF AMMONIA ON CHLOROPHYLL-BODIES. 281 
solution of osmic acid. Nor, again, in a leaf which had been 
immersed in an infusion of raw meat for 24 and for 50 hours : 
and in this leaf the chlorophyll-grains were still visible in many 
places, but were sometimes heaped together. N otwithstanding 
the diffieulty of ascertaining the effects of carbonate of ammonia 
on the chlorophyll-grains, chiefly owing to the action of water 
on them, I am led to believe, from the gradations which could be 
followed, and from the absence of chlorophyll-grains in the cells 
in which one or two large spheres were present, that in the case 
of the palisade- and parenchyma-cells matter produced by the 
disintegration of the grains first aggregates, together with other 
matter derived from the cell-sap, into minute globules, and that 
these aggregate into the larger spheres. I will give a single 
instance :—A leaf was immersed for 221 hours in a solution of 
the carbonate of 4 to 1000, and sections, after being cleared in 
alcohol, exhibited in many places distinct chlorophyll-grains, and 
in other places only very fine granular matter, and in avery few 
cells minute transparent globules. The leaf was left for 24 
additional hours in the solution; and now sections cleared in 
alcohol exhibited numerous minute shining translucent globules, 
many of which were smaller than the few remaining chlorophyll- 
grains. There were also other much larger transparent spheres, 
more or less confluent, which, when irrigated with acetic acid, 
instantly disappeared. 
A leaf was immersed in a solution of 4 parts of phosphate of 
ammonia to 1000 of water, and after 23 hours there was no trace 
of aggregation. It was left for 244 additional hours in the solu- 
tion ; and now sections cleared in alcohol exhibited not only 
minute shining colourless globules, smaller than the few remain- 
ing chlorophyll-grains, but plenty of large spheres, more or less 
aggregated together; and in the cells containing such spheres 
no chlorophyll-grains could be seen. The spheres, both large 
and small, disappeared instantly when acetic acid was added, as 
ın the case of those produced by the carbonate. It appears, 
therefore, that these two salts act in the same manner, but that 
the phosphate acts more slowly than the carbonate, as is like- 
wise the case with Drosera. A leaf immersed for 45 hours in a 
solution of 2 parts of nitrate of ammonia to 1000 of water was a 
good deal infiltrated and darkened in colour; but no spheres 
were formed; some of the chlorophyll-grains had, however, 
become confluent while still adhering to the walls of the cells. 
