BET. K. ABB AT OS HEMILEIA YASTATRIX. 177 



ramifies amongst the cells of the leaf. The tendency to form 

 little sacs full of granular matter is noticeable, as is the fact 

 that two of the dark bodies are connected by one branch of 

 mycelium. 



After examining mauy hundreds, I may truly say thousands, 

 of these clusters of sporanges, I had the good fortune to break 

 open the sides of a stomate and detach the perfect fungus from 

 the cuticle. A glance showed the cause of all previous failures. 

 The parts of the fungus within and without the leaf were seen to 

 be connected by a narrow neck, which always broke when any 

 attempt was made to detach the clusters or the dark bodies from 

 the cuticle. PI. XIII. fig. 4 shows the fungus thus detached — A, 

 being the cluster of sporanges outside the leaf; B, the dark body 

 filling the whole of the intercellular air-chamber behind the sto- 

 mate ; C, the mycelium attached to and carrying nutriment to B. 

 Of the exact nature of the dark body B, I am unable to speak with 

 accuracy. It seems to be merely an enlargement of the myce- 

 lium into the form of a sac of the shape and size of the air- 

 chamber it occupies, and filled with dark- red granular matter 

 conveyed to it by the mycelium-branches C. This view is partly 

 confirmed by PI. XIII. figs. 5 and 6, in which the tendency of 

 the mycelium to form little sacs full of red granular matter is 

 very decided. All these figures are from specimens obtained from 

 the interior of diseased coffee-leaves sent from Ceylon. Fig. 5, I 

 believe, shows one of the dark masses in process of formation, and 

 before it has pushed its way through the stomate. The general 

 character of the mycelium that permeates the intercellular spaces 

 of the leaf is seen in fig. 7 (PI. XIII.). Not infrequently, how- 

 ever, these branching masses of mycelium are so complicated that 

 it is almost impossible to represent them on a plane surface. In 

 one instance, in w T hich the immature form of fig. 4 (PI. XIII.) was 

 detached from the growing coffee-leaf, the dark body C was found 

 to be transparent and partially filled with red granular matter, 

 some of which was observed to be passing up the neck towards 

 the sporanges. 



"When one of the clusters of sporanges growing on the outside 

 of the leaf has been removed and placed between two slips of 

 glass, all the more mature sporanges may be detached by pres- 

 sure and only the immature sporanges left. These may be seen, in 

 PI. XIII. fig. 8, attached by short pedicels to a central dark-red 

 mass, which is no doubt the terminal portion of the dark body 



