BET. R. ABBAT ON HEMILEIA YASTATBIX. 181 



and after a time differentiates itself into one or more distinct 

 bodies each containing a nucleus. This is distinctly seen in 

 PI. XIV. fig. 17. In specially vigorous specimens these bodies, 

 at the end of sixteen or eighteen days, develop into bodies appa- 

 rently exactly similar in character and size to the spores from the 

 sporanges, the attachment of the spore being distinctly visible, as 

 also are the semitransparent nuclei (PL XIV. fig. 18). Most 

 frequently, however, the cells do not attain such a perfect deve- 

 lopment ; but they burst, and the minute granular matter passes 

 out without any further development into spores. 



When the growing fungus is fed with boiled and filtered coffee- 

 leaf juice, the granular protoplasm within the .cells assumes a red 

 tinge similar to that contained in the mycelium extracted from 

 diseased coffee-leaves. The tendency of some of the cells of the 

 mycelium, w r hether interstitial or terminal, to swell into a sac-like 

 form is very marked. They attain at times to the size of three 

 quarters of the red sporange, or about '00075 inch in length and 

 nearly the same in breadth. In one or two instances they have 

 presented an appearance suggestive of conjugation ; but at this 

 point the growth of the fungus ceased, and I was not able to 

 detect any actual communication between what appeared to be the 

 antheridium and the oogonium*. 



The conidioid form of fruit may very frequently be obtained by 

 growing the mycelium on glass slides, the complete form being 

 shown in PI. XIV. figs. 19 and 20. These conidia are somewhat 

 obovate and, when mature, papillated. Under a high power a 

 fine connexion may be detected uniting together neighbouring 

 conidia. In one instance (PI. XIV. fig. 20) the terminal coni- 

 dium was very much larger than the others, and remained per- 

 fectly smooth whilst they were papillated. These conidia are 

 about '00015 inch in length, and when placed in water germinate 

 readily, producing a mycelium which is generally free from septa ; 

 but in some instances one or two septa are distinguishable 

 (PI. XIV. fig. 21). They also produce a second generation of 

 conidia similar, but of inferior vitality, to the original ones. 



With regard to zoospores, I have several times found them on 

 the slips under circumstances that suggested a connexion with 



On this head consult the Eev. M. J. Berkeley's "Observations on a pecu- 

 liar mode of Fructification in Chironyphe Carterii" Journ. Linn. Soc. vol. viii 

 p. 139, tab. x. figs. 1-3. 



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