466 ON THE GEMMJE OF AULACOMNION PALUSTRE. 
in water; in the latter case, 
however, only protonemal fila- 
ments were formed. 
When sown on damp soil, 
normal protonemal filaments 
were found to be already deve- 
loped from single cells of the 
gemma on the third day. 
Apparently any external 
cell may grow out into a pro- 
tonemal filament, their forma- 
tion being restricted to no 
special part of the gemme 
(figs. 3 and 4). After the 
protonema has attained a 
strong development, a leaf- 
forming bud appears in the 
ordinary way upon it, at a 
point close to the gemma 
itself, and in no definite posi- 
tion relatively to it. 
Fig. 3. 
Gemmule of A. palustre, after germination 
for three days. 
Gemmation of 4. palustre in a further stage of development. The shaded fila- 
ments are green protonema, a leaf bud is formed near the base of one? 
them. The filaments which are not shaded are rhizoids. 
