214 
authorities took this species for an organism and others did not. 
Woronin was the first to place it among living organism, the 
bacteria. Beyerinck believed that they were modified bacilli. 
Ward maintained that they were the budded-off gemmules or 
spores of a true hyphal fungus, as already explained. Brunchorst, 
and also Frank, said they were bacterioid collections of albumi- 
nios substances formed from the cell protoplasm. Recently 
Frank claims that they are true R/izobia, because plasmolysis 
causes them to contract like living protoplasm. 
I have found this species symbiotic with Trifolium pratense 
and 7. repens, Melilotus alba and Lathyrus odoratus. It is at 
once recognized by its size and the varying form it takes on 
during the vegetation period. It was impossible for me to detect 
the presence of spores during any time of its existence. It very 
likely multiplies by division only. The tubercles produced are 
oblong or irregular, varying in size from that of a pin head to that 
of a small pea; sometimes they are collected in large grape-like 
bunches, as in Me/ilotus alba. The modified organisms do not 
develop upon gelatine or agar-agar cultures. They are surround- 
ed by a gelatinous zone. 
Rhizobium curvum, n. s. 
' Syn: Rhizobium leguminosarum, Frank. | 
Phytomyxa leguminosarum, Schroeter. 
_ Cladochytrium leguminosarum, Vuillemin. r 
Bacillus radicicola, Beyerinck. 
Pasteuriacia, Laurent. J 
Symbiotic with Phaseolus pauciflorus, Cells curved, resembling 
the comma bacillus of Koch. One hemispherical spore at each 
end, rarely more. Size quite uniform, and form constant during 
entire vegetative period. Gelatinous zone present. Tubercles 
spherical, varying in size from that of a pea to that of a small 
hazel nut. 
in par 
Rhizobium Frankii, n. S., Var. majus, N. V. 
Syn.: Same as preceding. 
Symbiotic with Phascolus vulgaris, Cells straight, generally 
two or three times as long as broad. Size variable. Two sphe™ 
ical spores, one at either end, sometimes one, rarely none. aise 
