134 



MISSOURI BOTANICAL GARDEN. 



th 



copper sulphate. 



In the case of the intumescences formed on cauliflower 

 in the Missouri Botanical Garden, the f™tnr« nf mmof,,™ 



heat 



ght played absolutely no part whatever. As 

 stated above, in no case were any intumescences formed 

 except as the immediate result of spraying with various 



fungicides. 



ho wed 



which formed on tli 



~ • - — ™ — ■ ^ m^ ■*--* ^ « \ m r^J V> \J IX 



sprayed with a standard solution of copper ammonium car- 



l^^ A. - l_ _ 1 • i m 



had 



added. No intumescences appeared on the leaves sprayed 

 with Bordeaux mixture or potassium sulphide, the other 



^ _ _ _ . T > it . • 



d 



It would appear from this that 

 the intumescences must have been caused either by the 

 copper ammonium carbonate or by the glue, or by'some 

 substance formed as a result of the combination of copper 

 ammonium carbonate and the slue. In o 



do 



one of the substances brought about the formation of the 

 intumescences a number of thrifty cauliflower plants were 



ed 



Plant No. 1. 



form. 



Plant No. 2. 



Four leaves sprayed with flsh glue in its concentrated 



Two leaves sprayed with flsh glue diluted 50% with water 

 Plant No. 3. Four leaves sprayed with Bordeaux mixture, 6 lbs 

 CuSOi, 4 lbs. lime and 50 gallons water. 



Plant No. 4. Four leaves sprayed with Bordeaux mixture to which 

 about 1/3 glue was added. 



Plant No. 5. 

 Plant No. 6. 



Four leaves sprayed with ammonium copper carbonate 

 Four leaves sprayed with ammonium copper carbonate 

 to which about 1/3 glue was added. 



The plants were sprayed about twelve o'clock and in the 



Tne s P ots of Bordeaux mixture and ammo- 

 nium copper carbonate dried in about two hours. Five 

 days after the spraying the following condition was noted : 



bright sun. 



Plant No. 1. With 



The tissue under the glue spots had 



shrunken and died, both on the upper and lower surface of the leaves 

 There was no sign of oedematous tissue under or near any of the spots.' 





