CHROMOGENIC FUNGI WHICH DISCOLOR WOOD. 85 
A. Sprecies oF GRAPHIUM WITH A SECONDARY CONIDIAL 
STAGE WITH CONIDIA RESEMBLING THOSE OF SPORO- 
TRICHUM. 
Graphium ambrosiigerum n. sp. 
A species of Graphium was found growing in the gal- 
leries of wood-boring beetles in the wood of Pinus Arizon- 
ica Eng. This material was furnished by Dr. A. D. Hop- 
kins in connection with the study of the relation between 
ambrosia fungi and ambrosia beetles, a subject which is still 
under consideration in connection with the study of such 
beetles by Dr. Hopkins. Pieces were taken from the 
stained wood in the interior of the block near a gallery, 
and placed in test tubes of pine sapwood, which had been 
sterilized after being moistened. From the mycelium in 
the stained wood there grew out a white growth, bearing at 
first the secondary then the primary conidia of Graphium. 
Secondary conidia were found in some of the galleries 
when the material was first examined, but were not recog- 
nized as such at the time. The following are the cultural 
characters of the fungus :— 
MYCELIUM AND SECONDARY CONIDIA. 
When cultures are made with either the primary or sec- 
ondary conidia on pine decoction agar, the spores generally 
germinate ina few hours, and in two or more days colonies 
may be noted with a white, sparse mycelium. At this 
stage of growth, secondary conidia appear of the Sporotri- 
chum-type, borne on erect, simple hyphae (pl. 8, f. 5}. 
These are hyaline, and oval in shape. After a few days, 
portions of the mycelium assume a brownish color, and 
from certain matted masses of filaments or stromata there 
grow out tufts of erect, parallel hyphae (pl. 8, f. 6). 
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