FUNGOUS ROOT-TUBERCLES. 05 



made a cytological study of the endotr 



m 



rhizome of Psilotum 



trum. In the Podocarpus tubercle; 

 mvcete, which, by branching, filled 



host 



olume and assumes an amoeboid 



whereupon it divides mitotically, frequently forming as 



distributed 



the cell and again become amoeboid in form. When the 



fungu 



resume normal 



mitotically. No cell walls are formed subsequent to nuclear 

 division, the cells ultimately degenerating with the disinte- 

 grating tubercle. Regarding the symbiotic relationship, 

 Shibata corroborates the views held by Magnus (22) and 

 Frank (10), who have shown that the f 

 to the host cell. Shibata showed further 



by 



the tubercle. 



enzyme capable 



rhizome of Psilotum 



similar 



m connnes itseii 10 una penpueij ui mc **>**, c 

 cleus undergoes no division. The undigested 

 > f uncus form a dense aggregation in the cente 



mented 



membrane. This is quite simil 



to the conditions in Neottia Nidus avis. By means of descrip- 

 tions and figures, Shibata presents the true conditions as 

 they are found in the tubercle of the alder. Here in addition 

 to what earlier investigators had found, he mentions a dense 

 clump of cytoplasm which remains after the absorption of 



fungu 



term 



bodies 



cells of several animals. These he claims are instrumental 

 dn the production of an enzyme in the cell which dissolves 



the fungus. 



Bjorkenheim (2) in one of the most recent papers on the 



alder tubercles presents the old facts in a somewhat different 





