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MISSOURI BOTANICAL GARDEN 



light without, however, contributing anything new. He finds 

 very large hyphae in the young tubercles, some measuring 

 4-5/4 in width, which become finer as the tubercles grow 

 older. His other facts regarding the fungus and the host cell 

 are similar to those described by earlier investigators. 



Wolpert (54) made a study of the tubercles on Alnus 

 alnobetulina and corroborates the results of Brunchorst and 

 Shibata, in addition showing intercalary and apical swelling 

 of the septate hyphae, conditions which are similar to the 

 enlarged hyphae found in Psilotum. A view not earlier 

 advanced is that the angular structures in the sporanges ger- 

 minate and form new hyphae, but from the text figures given 

 it would appear to be an artifact rather than an actual ger- 

 mination. The so-called "Secretkorperchen" described by 



Shibata were not found in those cells where the fungus is 

 digested. 



THE TUBERCLES OF CEANOTHUS. 



For cytological study the root-tubercles of Ceanothus 

 americanus were gathered from plants growing in the open 

 woods surrounding Madison, Wisconsin, the material for sub- 

 sequent investigations being collected from plants growing 

 in Forest Park, and in other open woods on the outskirts of 

 St. Louis, Missouri. Because the tubercles dry very easily 

 on exposure, fixations w r ere made in the field in order to 

 obtain as nearly perfect material as possible. A few plants, 

 however, were removed from the soil and transplanted into 

 flower pots which were kept in the greenhouses, where the 

 roots were kept in good condition for a reasonable time, 

 packed in a large amount of moist sphagnum. 



Judging from the material gathered at various sections of 

 a state as well as from different states the indications are 

 that the species of Ceanothus are everywhere affected with 

 tubercle growths. Ceanothus americanus and ovatus, com- 



mon 



micro 



States, which are now growing in the Missouri Botanical 

 Garden, were all examined and found to possess tubercles. 



