92 



MISSOURI BOTANICAL GARDEN. 



3. Extract which had been heated for ten 



minutes at 



80° C. 



(a) At end of 24 hrs.— No digestion, liquid clear. 



(b) " " 



(c) " " 



" 48 

 " 72 



No action, " 



Fibrin still unaltered. 



4. The control, with glycerin and aqueous extracts of root 



tissue, was placed under the same conditions as the above, 

 but no indication of direction w«« chniim 



Experiment II. 



Distilled water extract was made of alder tubercles. To 

 50 cc. of extract, .25 gram of fibrin was added. Tempera- 

 ture 34° C. 



1. A normal aqueous extract. 



(a) At end of 48 hrs.— Half of fibrin digested. 



(b) 

 (c) 



i( 



it 



" 72 

 " 96 



i i 



it 



More digested, liquid turbid. 

 All digested. 



2. Extract with 5 cc. of 1% IIC1. 



(a) At end of 48 hrs.— Considerable digested. 



(b) " ■" 



(c) " " 



" 72 

 " 96 



Fibrin nearly digested, liquid turbid. 

 Fibrin all digested. 



3. Extract with 5 cc. 1% Na 2 GO,. 



(a) At end of 48 hrs.— Liquid clear, no digestion. 



(b) " " 



(c) " " 



" 72 

 " 96 



Fibrin hardened, no digestion. 

 No digestion. 



4. Extract heated to 80° C. 



(a) At end of 48 hrs. 

 " " "72 " 



" " " 96 " 



(b) 

 (c) 



No reaction. 

 No reaction. 

 No change in fibrin 



Experiment III. ' 



Glycerin extract of alder root tissue. To 50 cc. of extract 

 .25 gram of fibrin was added. Temperature 34° C. 



1. Normal extract. 



(a) At end of 24 hrs.— No reaction. 



(b) 



(c) 



(d) 



4( ii ti ijn n 



i i 



it 



a 



96 



t t 



Fibrin shriveled. 

 No digestion. 



No reaction for digestion 



