FUNGOUS ROOT-TUBERCLES. 



93 



2. Extract with 5 cc. 1%HC1. 



No change in fibrin could be noted at end of 96 hours, and 

 the filtered liquid gave only a slight biuret test for proteids. 



3. Extract with 5 cc. 1% Na 2 C0 3 . . 



No reaction for proteids at the end of 96 hours. 



4. Extract heated to 80° C. 



oted 



The above data indicate that the greatest amount of fibrin 

 is digested in a weak acid solution and at the higher tern- 

 perature. 



was 



heated and 



Experiment IV. 



An aqueous extract of alder tubercles was used to deter- 

 mine the amount of fibrin 50 cc. will digest in 24 hours at 

 20° and 43° C. The fibrin was thoroughly dried before each 

 weighing and then soaked in 1% HC1. before it was used. 



Extract. 



1. Normal. 



2. With 5 cc. HC1. (1%). 



3. 



4. 



With 5 cc. Na 2 COs (1%) 

 Heated to 80° C. 



1. Normal. 



2. With 5 cc. HC1. (1%) 



3. With 5 cc. NaaCOs. 



4. Heated to 80° C. 



At 20° C. 

 Original wt 

 .25 g. 

 .25 » 

 .25" 

 .25 " 



At 43° C. 

 .25 g. 

 .25 " 

 .25 " 

 .25 " 



Experiment V. 



With extract from root tissue. 



Extract. 



1. Normal. 



2. With 5 cc. 1% HC1. 



3. With 5 cc. 1% Na 2 C0 3 . 



4. Heated to 80° C. 



Similar experiments were carried on wi 

 43° C, but no loss of weight was obtained. 



At 20° C. 



Original wt 

 .25 g. 

 .25 " 

 .25 " 

 .25 " 



After 24 hrs. 

 .249 g. 



.23 

 25 

 25 



it 



ii 



< ( 



217 g. 

 ,115 " 

 249 " 

 25 " 



After 24 hrs 

 .25 g. 

 .25 '* 



.25" 

 .25 " 



Loss. 



.001 g. 



.02 " 

 No loss. 

 No loss. 



.033 g. 



.135 " 



.001 " 



No Iosb. 



Loss. 

 None. 

 None. 

 None. 



None. 



I 



