94 



MISSOUBI BOTANICAL GARDEN 



Experiment VI. 



With aqueous extract from Ceanothus tubercles, .25 gram 

 fibrin was placed in 50 cc. < 



f extract which was kept 



33° C. A drop of 



Extract 

 1. Normal. 



dded 



At end of 7 hrs. 



After 24 hrs. 



Slight action— liquid Some digested, 

 becoming turbid. 



2. With 5 cc. 1% HC1. Apparent digestive Fibrin becoming filled 



action. 

 3. With 5 cc. Na 2 C03. Liquid dark, fibrin 



with bubbles. 

 No action on fibrin. 



4. Heated to 70° C. 



The same extract. 



contracted. 

 No action. 



A slight precipi tate. 



1. Normal. 



At end of 48 hrs. 

 Fibrin digested, 

 liquid turbid. 



After 72 hrs. 

 Half of fibrin digested. 



2. With 5 cc. 1% HC1. One-half of fibrin 





digested. 



3. With5cc.l%Na 2 C0 3 . No digestion. 



4. Heated to 70° C. No action. 



Only a few small pieces 



left. 

 No digestion. 



No action. 



After 48 hours of digestion some filtered liquid from each 

 flask was tested for proteid. The solutions from 1 and 2 

 gave good biuret tests, indicating further that normal and 

 acid extract will digest fibrin. No proteid reaction could be 

 obtained from 3 and 4, showing that the enzyme was de- 

 stroyed under the conditions or else its action was inhibited. 

 After six days all the fibrin in 1 and 2 was digested. 



Experiment VII. 



To 50 ccc. of dilute glycerin extract of root tissue of 

 Ceanothus .25 gram of fibrin was added. A drop of chloro- 

 form was added as an antiseptic. Temperature was 23° C. 



Extract. 

 1. Normal. 



After 7 hrs. 



24 hrs. 



72 hrs. 



Liquid clear. No digestion. No digestion. 



2. With 5 cc. 1% HC1. No digestion. No digestion. No digestion. 



3. With 5cc. 1% Na 2 C03. No digestion. No digestion. No digestion. 



4. Heated to 80° C. No digestion. No digestion. No digestion. 



Judging from these results no enzyme which will digest 

 fibrin is present in the root tissue of Ceanothus. 



