embryo; seed and carpel in the date. 139 



period. Toward the close of the second period, although 

 there is pressure upon the endosperm surrounding the 



em 



there is still no digestion of the cell walls 

 to be "noted. There is therefore no breaking down of tissue. 

 During the early part of this period, however, there is to be 

 seen the independent action of the embryo in the unequal 

 distribution of oil in the surrounding endosperm (f. 14). 

 At this moment the accumulation of oil throughout the latter 

 is marked, but much less so in a spherical mass surrounding 



the embryo. This sphere measures 



100 microns, more or less, from it. In addition to I 

 amount of oil in this region, I have noted that my 



some 

 small 



form 



show an accumulation of 

 26. 26a") . I have not seen 



it in the same or corresponding region in this form at any 

 subsequent time. The preparations in question were fixed 



ferric 



before and 



chloroform, so 



be 



tion. This appearance of tannin is synchronous with the 

 beginning of reserve cellulose formation, when, as already 

 stated, tannin appears in that portion of the endosperm 

 where the thickening of the walls occurs. It may therefore 

 very well be that this tannin is concerned in the nutrition 

 of the embryo, but my material was not sufficient to settle 



must 



com 



time 



bryo 



The appear- 



ance of the oil in the embryo differs from that in the 

 endosperm. In this, when the oil first appears, it occurs in 

 extremely minute droplets, which collect in a zone about the 

 nucleus. This is especially well shown by the pavement en- 

 dosperm cells lining the cavity (f. 9). In the embryo, 

 however, this relation is not to be seen. Here the oil occurs 

 anywhere in the cytoplasm, as depicted by Sachs in the epi- 

 thelium of the growing haustorium. 





