34 MISSOURI BOTANICAL GARDEN. 
They were harvested three weeks after the cultures were 
started. Spores were taken only from pure cultures of the 
fungus. ‘They were ground up in a mortar with fine quartz 
sand. Before being used this sand was boiled in alkali, then 
in acid, and finally in several changes of distilled water, in 
order to free it of impurities. The entire mass of spores after 
being ground up in this way, was subjected to a high pres- 
sure, and several cubic centimeters of sap were squeezed out. 
This sap solution was reddish-yellow in color, had a specific 
gravity of about 1.053, contained absolutely no reducing 
sugars, was neutral to litmus and gave, on the addition of 
alcohol or when heated above 50° C., a heavy floculent 
precipitate. 
Some spores and mycelial cells exactly like those used for 
obtaining sap were placed in this solution. After sixteen 
hours none of the cells showed plasmolysis; most of them had 
germinated and the solution was filled with mycelium. Cells 
of Spirogyra setiformis, on the other hand, became quickly 
plasmolysed when placed in the sap of the Monilia spores. 
Some of the sap solution was boiled down to one-tenth of its 
original volume. In this concentrated solution the spores 
and mycelial cells became quickly and permanently plas- 
molysed. The exact concentration of the sap that would be 
just sufficient to plasmolyse the cells was not determined, but 
it was found that even after considerable concentration the 
sap solution would not cause plasmolysis. This result in- 
dicates that the Monilia cells do not behave as ordinary 
osmotic systems; if they did we should expect them to become 
plasmolysed in solutions of their own sap, even when such 
solutions are only slightly more concentrated than the sap. 
In slightly concentrated solutions of their cell-sap the spores 
germinate and grow. From this it seems that spores of 
Monilia are able to take up water from solutions more con- 
centrated than their own cell-sap. 
As was mentioned above, the sap solution was of a reddish 
yellow color. It is interesting to note in this connection that 
when the spores are placed in the sap solution they readily 
take up the coloring matter and become a much deeper color 
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