A PARASITIC BOTRYTIS ON PEPPER AND LETTUCE. 55 
Tomato, pepper and apple broths gave about the same results, 
the mycelium growing equally well in all the media. Potato 
broth furnished a better medium, for, after the mat of my- 
celium was formed on the surface, conidia and sclerotia were 
produced in abundance. 
On a two per cent lettuce agar, growth was rapid for about 
two days, afterwards decreasing slowly. In seven days the 
medium was covered with a thin layer of mycelium, which 
then produced conidia. Only a few sclerotia, about the size 
of a pinhead, arose from the hyphae. A similar growth was 
obtained on apple, pepper and tomato agar. Growth on 
potato agar was more vigorous, conidia and numerous 
sclerotia being formed. The spherical sclerotia, as a rule, 
were larger than the others mentioned above, being the size 
of a small pea. 
Very little mycelium was obtained on nutrient gelatin and 
bouillon, no sclerotia and only a few conidia developing. 
When the study of the parasitism of the fungus was under- 
taken, a large number of cultures were made on sugars, fats 
and acids. The results of this work will be discussed under 
parasitism. 
Sterilized corn-meal, saturated with various juices, gave 
good results, inducing rapid development of sclerotia. Apple, 
lettuce, tomato, pepper, potato and prune juices were used. 
Corn-meal moistened with potato and prune juices furnished 
the best medium for growth. Later, sterilized bread-crumbs 
were substituted for the corn-meal, as it was found to produce 
mycelium much more abundantly. In all of the above cul- 
tures growth was not rapid at first, but in five days the 
mycelium covered the entire surface as a loose, white, cottony 
mass composed of large, branching, septate hyphae. Organs 
of attachment were produced in large numbers wherever the 
mycelium came in contact with the sides of the dish. 
The crowding of the mycelium led to the formation of a 
rather regular zone of sclerotia. As the cultures became older 
the latter united with one another, so that the zonate appear- 
ance was lost to some extent. The sclerotia matured in from 
