A PARASITIC BOTRYTIS ON PEPPER AND LETTUCE. 59 
integrated and disappeared, none remaining intact. The 
large majority of them germinated, the others rotting within 
three months. 
Thinking that possibly the sclerotia could be induced to 
germinate otherwise than by conidia, various chemical agents 
were used to stimulate them. Sclerotia obtained from the 
above sources were used in these experiments. One series was 
placed in a saturated atmosphere of chloroform for twenty- 
four and forty-eight hours. This was repeated for ether. 
Others were placed in desiccators, where they remained from 
one to seven days. The sclerotia were then placed in flasks 
as before. 
Eighty-five per cent of the sclerotia placed in chloroform 
and ether vapors and fifty per cent of those exposed for forty- 
eight hours formed tufts of conidiophores. Germination, as 
before, was rapid. The cultures were allowed to run for three 
months, but no further development was observed. Conidia 
obtained from all the flasks and placed on media showed no 
differences in growth and development. Sclerotia, when 
mature, placed on moist sand, again formed the characteristic 
tufts of conidia. Careful observations in the field and green- 
house showed no further development of the sclerotia other 
than the production of conidia. About April 15, the 
sclerotia placed in the field formed conidiophores. 
From the numerous experiments outlined above it follows 
that the fungus, Sclerotinia Fuckeliana, with Botrytis cinerea 
on lettuce as its imperfect stage, has lost its apothecial stage 
entirely, the life cycle being complete when the conidia, pro- 
duced by the sclerotia, germinate to form a vegetative my- 
celium. 
Germination of the conidia: The conidia germinate readily 
in water and nutrient solutions. Germination by one, some- 
times two, germ tubes is the rule. In two days the mycelium 
is quite profuse and if the germinating spores are in hanging 
drops, organs of attachment are formed almost immediately. 
The conidia from the sclerotia germinate in like manner. 
Three generations of conidia have been observed, no difference 
