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A PARASITIC BOTRYTIS ON PEPPER AND LETTUCE. 65 
solution and extract, no iodine reaction could be obtained after 
an hour, although Fehling’s solution gave negative results. 
However, at the end of twelve hours, hydrolysis had taken 
place and the final product appeared to be dextrose. The 
slow growth on the starch medium at first, also shows that 
hydrolysis must take place before it can be digested by the 
hyphe. A three per cent solution of dextrose gave a vigor- 
ous growth from the beginning. The mycelial development 
was luxuriant, many sclerotia and some conidia being formed. 
Growth on cane sugar like that of starch was rather slow 
at first. By the end of the first week the development equaled 
that in the dextrose culture. Tested in the usual manner, a 
strong reduction was observed, showing that inversion had 
taken place. As before, the extract produced similar changes 
in the medium. 
Sugar solutions of various concentrations were inoculated 
with Botrytis conidia. Growth resulted in all cultures, the 
twenty and thirty per cent solutions giving the best develop- 
ment. Delicate tests failed to show the presence of any 
oxalic acid whatever. 
With maltose an excellent growth developed, producing 
sclerotia and conidia. Lactose gave a fairly good growth 
after the first week. On levulose an abundant mycelium, 
similar to that on dextrose, developed. On dextrin, growth 
was slow at first, but soon a vigorous mycelium was pro- 
duced. A strong reduction was noted in the cultural solu- 
tion and in the medium after treatment with the extract. 
In nearly all cases, Fehling’s solution shows the presence 
of reducing sugars in originally non-reducing carbohydrate 
media upon which Botrytis has been grown, showing that 
a number of enzymes are secreted by the mycelium. No 
oxalic acid is formed in the cultures, contrary to Smith’s 
(49) results. Careful observations were made from time to 
time, but in no case was alcoholic fermentation detected. 
On glycerin, normal conidiophores were not produced. On 
testing for oxalic acid negative results were again obtained. 
