VOLATILE ANTISEPTICS AND SOIL ORGANISMS. 153 
tions, even when their work was duplicated as nearly as 
possible, that a cause was looked for in the period at which 
analyses were made. While in their work it is not stated 
whether analyses were made before or after evaporation of 
the chemical, presumably it was after. To determine this 
point an experiment was made which gave the following 
results :— 
Check not evaporated ........ 10.2 million. 
.2% Tol. not evaporated...... 41.6 - 
2% “after evaporation....00.56 “ 
As to whether this is the explanation, the writer is unable 
to say. 
Russell (30) further states that ‘many if not all the 
active forms of bacteria are also killed” by the treatment. 
To determine whether this were true, even when a decrease 
was noted, analyses of a sample were made after treatment, 
but before evaporation, and again after evaporation. In the 
evaporation of this sample, the water content fell from 10% 
to 8%, and in Table IV is given the percentage decrease 
due to this loss of water. 
TABLE IV. 
SHOWING THE PERCENTAGE REDUCTION IN NUMBER OF BAC- 
TERIA AND ACTUAL REDUCTION IN TYPES OF 
PROTOZOA DUE TO EVAPORATION OF 
CHEMICAL AND MOISTURE. 
Protozoa Present 
Treatment 7%, Hal ae 
siicecabal Before After 
Check 48 6 7 
CS, 0.2% 68 6 5 
CS, 1.0% 60 4 3 
CS, 4.0% 72 0 0 
Tol. 0.2% 61 3) 3 
Tol. 1.0% i 0 0 
Tol. 4.0% 73 0 0 
CS2 1.%, CS2 4.%, and Tol. 4% showed a decided decrease 
in number of bacteria during exposure to chemical. 
