154 MISSOURI BOTANICAL GARDEN. 
If all vegetative cells were killed by the chemicals in ques- 
tion, a reduction of the per cent of water from 10 to 3 in 
the short space of 48 hours should not give us a decrease 
in number of organisms. From these results and conclu- 
sion (5) above, it seems evident, that even when there is 
a reduction it only affects certain species and not the vege- 
tative cells of all species. This is in accord with the results 
of Hiltner and Stérmer, and in part with those of Russell 
and Hutchinson in that they found quite a difference in 
the types of organisms subsequent to treatment. 
Greenhouse Experiments. 
In Table V is given an excellent and typical example 
of the effect of the substances studied on the number of 
bacteria in soils under greenhouse conditions. 
TABLE V. 
SHOWING THE BACTERIAL CONTENT OF A SERIES OF GREEN- 
HOUSE PLATS DURING THE GROWTH PERIOD OF A 
CROP, WITH THE RELATIVE YIELD 
OF THE SAME, 
Date | 2-21 | 2-25 3-4 | 3-11 | 3-18 | 4-3 | 4-18 | 5-10 || Zotel | Grain 
Mae... 81| .63} 5.50] 4.60! 1.60] 2.70/ 4.10/ 200/| 100 | 100 
Bterilixed...:| ‘O0|..... 130.00|174.00|106.00|102.00| 50.00| 83.00|| 155 | 213 
Mec... 12 |'1.90 | 39.00] 91.00] 42.00/106.00|141.00/116.00|| 209 | 259 
T. 22.5cc....| '81| :74| 2:40] 2:50] .74| 3.401 4.00] 4.00l| 102 
T. 62. cc....| .81| 1.50] 3.10| 1.00| 2.10] 4.00/ 2:20] 200]] 123 | 158 
T. 310. cc....| .81| .75| 5.40| 5.40} 2.10| 6.70| 7.10] 2:70] 141 | 180 
“GOO eee 81| .63| 5.50] 1.60 2.20| 3.30] 1.20!| 100 100 
Sterilized. 00|..... 130.00|140.00| 74.00|163.00/159.00| 35.00|| 79 36 
RRR .12 |"1.90'} 39:00] 68:00] 22:00] 86.00| 74.00] 38.00|| 136 68 
C82 22.5cc.| '81/1:30| 3.001 85| 4.401 4.20/ 2.00\| 113 | 119 
CS: 62. cc.| ‘81| .93| \.70| 1:7 3.90 2.90| 1:80|| 144 | 150 
C82 310. cc.| (81| :73| 3:90] 1: '68| 3.50| 5.80} 6.70|| 73 
CHCls 62.cc.| ‘81|..... 1.00} :53| 1.001 2:60] 2:90|......].......|....0.. 
Figures on left of double line = bacteria in million; those on 
right relative yield. Plats above line seeded to oats, those below to 
buckwheat. T.=toluol. Pt. St. = partially sterilized. 
This experiment consisted of twenty-five plats 1x3 feet 
in size and six inches deep. They were filled with a 
pasture soil (as poor as could be had) and treated in fours, 
