156 MISSOURI BOTANICAL GARDEN. 
beneficial results. Similar toxic effects have been reported 
by Lyon and Bizzell (31), Schulze (32), and Picker- 
ing (33). 
TABLE VI. 
SHOWING THE PRESENCE OF DIFFERENT TYPES OF PROTOZOA 
AND NUMBER OF BACTERIA FOLLOWING TREAT- 
MENT OF GREENHOUSE PLATS. 
Treated Types Protozoa Present 12-15-’11 | Bacteria Million 
12-7-"11 |a/b;c|{dje|f/g{/h|i}j|k}| Total | 12-15 | 12-29 
Check....... + ere ee] eee el 9 9.0 7.0 
CS, 37cc +\—|+]/ +/+} +]/+/+/—]+i-| 8 13.6 11.6 
Tol. 37cc sad fee fat Ged fool oad Gad oe fe 9 10.8 11.2 
CS, 350ce. ..|+|—} +) +] +] +} +} +]+ 8 17.4 15.7 
Tol. 350cc. ..| ++) +/+) +/+}/+/+/) +/+ 9 13.7 19.5 
+ = present; — = absent. 
In Table VI is given another series of greenhouse plats 
in which soil was taken from an uncultivated orchard and 
was by no means a poor soil. The weaker application 
represents approximately 10 cc. per square foot, while the - 
heavier represents approximately 100 cc. per square foot, or 
3%. In both cases the antiseptic was mixed in the soil 
when applied. Here we have an unmistakable initial 
stimulative effect on number of bacteria. These plats are 
still under observation. 
From these results the conclusion seems justifiable: That 
under the experimental conditions given above the chemicals 
studied in quantities equivalent to 100 cc. per square foot, or 
1.%, do not exert a diminishing effect wpon the number of 
bacteria, but may or may not exert a stimulative effect. 
Field Experiments. 
About May 15, 1911, four series of plats (S. I, S. II, 
8. ITI, and S. IV), comprising a total of 140 plats, were laid 
out in the Missouri Botanical Garden. Each plat measured 
5x10 feet and was surrounded by a trench one foot wide 
