VOLATILE ANTISEPTICS AND SOIL ORGANISMS. 157 
and five inches deep. The plats in 8. I, 8. II, and S. III, 
were treated in duplicates of seven, as follows: check, 
toluol 1 cc. per square foot, toluol 15 cc. per square foot, 
CS2 1 cc. per square foot, and CSe 15 cc. per square foot. 
S. IV was subdivided into three series and treated as fol- 
lows: (a) in duplicate, check, toluol 1, 5, 10, 15, and 
30 cc. per square foot; (b) singly, check, CS2 1, 5, 10, 
15, and 30 ce. per square foot; (c) in triplicate, check, 
toluol 1 and 15, and CS: i and 15 ce. per square foot. The 
chemicals were added in one hole per square foot punched 
to the depth of five inches. 
S. I was seeded to oats; S. II to buckwheat; S. III to 
corn; and S. IV to millet. 
Unfortunately, no examinations of soil for bacteria were 
made until August 19, hence the initial effects are unknown. 
Table VII contains the results of such examinations as were 
made. Samples for these analyses were taken two or three 
in number from each duplicate plat and treated, as pre- 
viously given. 
TABLE VII. 
SHOWING THE NUMBER OF TYPES OF PROTOZOA AND NUMBER 
OF BACTERIA IN FIELD PLATS THREE TO 
FOUR MONTHS AFTER TREATMENT. 
Types of Protozoa Found 
ara a ett Tol. cc. Per Sq. Ft. CS, cc. Per Sq. Ft. 
Sampled Chk.| 1 | 5 | 10/15] 30] Chk.}] 1 | 5 | 10] 15] 30 
S.II 8-— 9’11...| 9 | 8].../...] 9 2 ari bate ot! 2 |r 
S. III 7-28-’11...| 6 ag ol loreal | ts sea [eee ies See roe as 
S.IV 8-31-11... 7 |7}/8)}8/[9/8] 8 | 7/7/,7],71{7 
S.IV 9-11-’11...} 9 |8]/8/8/9/8] 9 |9|7/]8|9)7 
Total No. Types..| 9 | 8|}8/}8/}91]8 9 |/9|7);8;9|7 
