VOLATILE ANTISEPTICS AND SOIL ORGANISMS. 159 
A third series was treated on the College Farm, Univer- 
sity of Missouri, and a record of treatment together with 
results of examinations made thus far is given in Table IX. 
TABLE IX. 
SHOWING NUMBER OF TYPES OF PROTOZOA AND NUMBER OF 
BACTERIA IN FIELD PLATS FOLLOWING TREATMENT. 
Plats 
Treated Tol. cc. Per Sq. Ft. CS, ce. Per Sq. Ft. 
11-8-’11 
Sampled |Chk.} 1 10 | 25 | 50 |Chk.| 1 10 25 50 
Types of Protozoa Present 
11-15-’11 9 7 9 9 9 9 6 7 8 7 
12- 1-’11 11 11 10 9 7 12 10 11 6 8 
12-14-11 11 10 9 9 8 9 10 7 7 8 
Bacteria Per Gram Soil 
11-15-’11 | 14.0] 14.2 | 18.9] 16.8 ].....| 14.0 | 16.0 | 15.6 | 10.0 | 13.3 
12— 1-11 | 15.6] 14.2 | 18.0] 18.6 | 15.3 | 19.2 | 14.3) 14.2]..... 39.9 
12-14-11 5.2} 4.9] 6.4] 7.3/23.5| 6.0] 5.4] 5.7] 6.7] 6.8 
Figures for bacteria equal million. 
From these results the conclusion seems justifiable: That 
while the chemicals used may exert a slight stimulative 
effect upon the multiplication of bacteria subsequent to 
treatment, all effects of this action were, in the soil studied, 
lost within three months time. 
