a little moldy copra together with a beaker of water to insure an abun- 
dance of moisture. They were then allowed to stand at room temperature 
(25° to 80° C.) for two months. 
as follows: 
After standing one 
Their change in acid value is shown 
After standing two 
| month. months. 
F. F. A. BR. BA, 1 oP BRA. F.F, A. -| 
| in oil in oil in | in oil in oilin | 
‘exposed to) original | exposed to) original | 
mold. bottles. | mold. bottles. 
Delsulonimes 0.22 0.16 | 0, 26 0.17 
0. 23 | 0.14 - 0.26 | 0.17 
Sisto wis dnduill 0. 22 | 0. 22 
0.18 | 0.88 | 
A slight increase of acidity was evident in each of these samples, but 
No. 11, an unfiltered, very turbid oil, was decomposed much more rapidly 
than either of the others. 
and taste and contained a visible mold growth. 
Since a marked difference was shown in the behavior of these oils, it 
was decided to observe the effect of the addition of small quantities of 
nutrient matter and of water on the rise in the free-acid contents of a 
pure oil. 
It also was characteristically rancid in odor 
The nutrient material which was used was prepared from 
“Jatic,” a coagulated residue produced in the native process of making 
oil, by boiling down an emulsion of fresh coconut meat. ‘This residue, 
when dried and extracted with chloroform, yields a light-brown powder, 
partially soluble in water and of a sweetish, not unpleasant, taste. It 
The following samples were 
consists chiefly of albumin and _ sugar. 
prepared, using pure, fresh oil as a base: 
A. 25 cubic centimeters oil + 0. 25 grams ‘‘latic.”’ 
B. 25 cubic centimeters oil -}- 0. 25 grams ‘‘latic’’ +- 0. 25 cubie 
centimeters water. 
C. Control of pure oil. 
Each of these samples was placed in a 50-cubie centimeter glass bottle 
inoculated with one drop of the moldy oil (No. 11) used in the previous experi- 
ments, and allowed to stand for one week at room temperature, and, after 
determining the increase in acidity, for one week in the incubator at 35°. 
Finally, the acidity was tested after the oil was allowed to, stand for two months 
longer at room temperature. 
. Initial 
Sample. acidity. 
| A 0.10 
B 0.10 
Cc 0.10 
One 
week at 
room 
tempera- 
ture. | 
| 
| 
One | 
week at 
B5° C. 
0.69 | 
0.11 | 
Two 
months at 
room 
tempera- 
ture. 
0.13 
