and some of the growth may adhere to the sides of the vessel; but the fluid is 
perfectly clear. The appearance presented is similar to that found in cultures of 
the Hrysipelas streptococcus. When a bouillon culture is shaken, the growth 
forms a stringy mass of grayish-white color; and the substance which arises from 
the bottom of the vessel is similar in appearance to that observed in bouillon 
cultures of Spirillum rubrum. The growth is quite sticky, and on being touched 
with the platinum wire it forms long strings, which are not easily separated. The 
organism does not ferment sugar. It grows in milk without coagulating the 
casein, 
After four days in gelatine stick cultures, kept at 18° C., there appear, along 
the line of the puncture, minute, whitish granules, which afterwards become 
larger and confluent. In three weeks the growth assumes a yellowish color. There 
is no liquefaction. 
On agar streak cultures there first develops a grayish-white growth, which in 
the course of time becomes yellowish. On agar tubes kept in the incubator, after 
twenty-four hours there develops a granular moist growth. If examined with a 
magnifying glass, the colonies are seen to be granular, the margin transparent, and 
the center dark-yellowish. 
On glycerine agar, the transparent margin is more marked and the develop- 
ment of the coccus is similar to that in common agar cultures. The development 
on urine agar is similar. 
On potato, a fine, light-yellow, dry deposit develops after twenty-four hours; 
this does not enlarge very markedly afterwards. 
On blood serum, after eighteen hours in the incubator, a very luxuriant growth 
develops in the line of the streak; this is shining, grayish-white in color and 
moist. After further time the growth spreads out from the streak in all direc- 
tions in the form of branches. (In culture media to which litmus has been added, 
no change of color occurs. ) 
Urine.—The morning urine of 34 patients was collected in sterile flasks. From 
these specimens culture media were inoculated. In 25 of these cases we succeeded 
in obtaining the coccus. 
Foces.—The feces from 44 cases were collected in sterile vessels and diluted with 
physiologic salt solution. We were successful in isolating the coceus on agar in 
15 of these cases. 
Animal experiments.—Twenty-one rabbits were inoculated; of these, 3 died. 
The first, inoculated with human blood, died twenty days after inoculation; the 
second, inoculated from the spleen of a white mouse previously inoculated from a 
pure culture, died nine days after inoculation; and the third, inoculated with 
cultures, died eight days thereafter. 
Seven guinea pigs were inoculated with human blood and with the heart’s blood 
and spleens of white mice. None of these died. 
Sixty-four white mice were inoculated, of which 17 died. Thirty-one of these 
were inoculated with human blood, of which two died; and 33 were inoculated 
from cultures, of which 15 died. 
Methods.—The injection into rabbits consisted of one tube of blood and sterile 
salt solution into the veins. In guinea pigs the same amount was injected intra- 
peritoneally or subcutaneously. In the case of the white mice, three-tenths of a 
tube (0.3 cubic centimeter?) was inoculated intraperitoneally, or else a platinum 
loopful of the pure blood was injected subcutaneously. One platinum loopful of 
agar culture mixed with sterile water was injected intraperitoneally into mice, or 
the same amount subcutaneously. The juice from the internal organs of animals 
dead of the disease was also injected.’ 
° The last part of the report is not clear, The translation here given is as literal 
as possible. 
