PART I, 
STUDIES UPON EXPERIMENTAL VACCINIA IN THE PHILIPPINE 
MONKEY (MACACUS CYNOMOLOGUS). 
1. VACCINIA FOLLOWING INOCULATION OF THE SKIN OF THE MONKEY. 
Lechmque.—Different strains of vaccine virus were employed. For 
convenience the different viruses will be indicated by Arabic numerals 
The source of each strain of virus is as follows. 
Virus 1.—Supplied by the Serum Laboratory of the Bureau of Government 
Laboratories,* Manila. A description of the method of its preparation will be 
found in the Third Annual Report of the Superintendent of Government La- 
boratories. 
Virus 148.—From the New York Board of Health. The pulp was mixed with 
60 per cent glycerin. 
Virus 246.—From the Japanese Imperial Board of Health. The pulp was mixed 
with 60 per cent glycerin which contained 1 per cent carbolic acid. 
Virus 236.—From the Vaccine Laboratory of Park, Davis & Co. The pulp was 
mixed with 60 per cent glycerin. 
Virus 251.—From the Laboratory of Chambon of Paris. The pulp was mixed 
with 60 per cent glycerin containing 1.5 per cent carbolic acid. 
We are indebted to Dr. J. J. Kinyoun, of Glenolden, Pennsylvania, 
for the last 4 of these viruses. He sent us a quantity of each of these 
strains and so arranged matters that, through the courtesy of the steam- 
ship company and of the United States Marine-Hospital Service, the 
virus was kept on ice from the time it left his hands until it reached us 
in Manila.® 
The skin of the abdomen was chosen as the site of inoculation. The 
hair was first shaved from a large area and the skin then scrubbed with 
soap and water, followed by alcohol. We have found the most satisfactory 
results to follow the inoculation of a shallow incision. As a rule a num- 
ber of such scratches were inoculated in each animal. When inoculation 
is done in this way, the study of the development of the lesion can be 
made to greater advantage than when scarification is practiced; for the 
lesion produced by the inoculation instrument is so slight that it heals 
“Now Bureau of Science. 
* We take this opportunity of expressing our gratitude to Dr. Kinyoun for his 
kindness in sending us these strains of virus and to those who assisted in forward- 
ing them. ‘ 
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