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Histological examination.—Both corneas present areas about 1 to 1.5 millimeters 
across, from which the epithelium is lost. In the center of this area the corneal 
connective tissue presents a minute defect representing the trauma of inoculation. 
The epithelium tapers to a thin edge about the denuded area and here are found 
eytoryctes in small numbers. Leucocytes are practically absent from the lesion, 
No. 20. Monkey inoculated on both corneas with vaccine virus No. 1, 
Twenty-four hours after inoculation both corneas are smooth. 
Forty-three hours. Slight distortion of reflected image. No conjunctival dis- 
charge and no photophobia. 
Three days. <A slight depression marks the line of inoculation, about which 
there is some roughening of the corneal epithelium. 
Four days. Marked roughening of cornea about inoculation sites with central 
loss of substance. Photophobia and some muco-purulent discharge noted. 
Six days. Condition same, save that the loss of substance is more marked. 
Animal chloroformed and tissues preserved for histological study. 
Histological examination.—Sections stained with eosin and methylene blue. 
Vertical sections taken just outside the edge of the area of the cornea denuded 
of epithelium show a widespread thickening of the epithelial layer and large 
numbers of cytoryctes at a point nearest the inoculation site. 
No. 17. Monkey inoculated on both corneas with vaccine virus No. 1. 
Twenty-three hours after inoculation both corneas are smooth, but the line of 
inoculation can be made out by the distortion of a reflected image. 
Fifty-three hours. Both corneas show slight loss of substance, together with 
some roughening. A slight degree of photophobia is present. 
Three days. The corneas are hazy and the loss of substance at the site of 
inoculation has increased. There is some photophobia and some muco-purulent 
discharge. 
Four days. Marked roughness of the corneal surface is apparent about the 
inoculation sites. Photophobia and discharge continue. 
Five days. Each cornea shows a shallow erosion, about which the cornea is 
rough and hazy. Photophobia and discharge still present. 
Six days. Condition same as yesterday save that the conjunctive are opde- 
matous, 
Seven days. Macroscopic appearances as before. Animal chloroformed and 
tissue saved for histological study. 
Histological eaamination.—The condition found is similar to that described in 
the other corneas of this series. There is an area about the point of inoculation 
over which the epithelium is wholly deficient. The surface is smooth and the 
corresponding portion of the cornea is slightly thinner than normal. 
The epithelium tapers at the border of the deficiency and at this point contains 
small numbers of cytoryctes. In one cornea there is a deep incision partially 
filled with epithelium. The tissue about this contains a small number of 
leucocytes. 
No. 10. Monkey inoculated on both corneas with vaccine virus No. 1. 
Twenty-four hours after inoculation the cornea appears smooth, 
Forty-eight hours. Corneas show slight distortion of the reflected image. 
Three days. Both corneas slightly roughened along the line of inoculation. 
Four days. Roughness is more marked and is present over a circumscribed 
area about the incisions. The cornea appears hazy and a small amount of muco- 
purulent material is present in the conjunctival sac. Slight degree of photo- 
phobia is observed. 
Five days. Condition same as yesterday, save that no photophobia is noted. 
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