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269 
corneal inoculations of the rabbit, in which the growth of the epithelium 
over compensates any destructive action. The removal of the degenerated 
epithelium was probably assisted by the dextrous rubbing of the eyes 
which was frequently observed with the monkeys inoculated upon the 
cornea. 
The course of development of the vaccinal lesion on the monkey’s 
cornea makes it a less favorable place than we had hoped for the study 
of the etiological factor of the disease. Owing to the loss of epithelium 
which takes away the bulk of the infected cells the sections are not rich 
in parasites. In spite of this, the sections from the corneal lesions yielded 
important data which will be considered in a later article dealing spe- 
cifically with cytoryctes variole. 
CONCLUSIONS. 
(1) Vaccination of the cornea of the monkey produces a lesion which 
is specific and which is comparable with that following the same inocula- 
tion in the rabbit. 
(2) The lesion is characterized chiefly by an early loss of epithelium 
at the site of inoculation, which is accompanied by the development of 
photophobia and conjunctivitis. 
(3) Cytoryctes vaccine are present in the cells of the lesion. 
3. VACCINIA FOLLOWING INOCULATION OF THE MUCOUS MEMBRANE OF 
THE MONKEY. 
In this section will be presented the results of inoculation of the 
mucous membrane of the monkey with vaccine virus. The evolution of 
the lesion at the site of inoculation, the general reaction of the animal, 
and the histology of the specific lesion will be described in some detail. 
These inoculations were undertaken to show what variations might result 
in the evolution of the specific lesion of vaccinia due to a change in the 
locus of inoculation. The material collected for the histological study 
of the specific lesion also served for the study of the parasite of the 
disease. 
Technique-—Nine monkeys were inoculated in this series. Vaccine 
virus No. 1 was rubbed into shallow incisions made on the mucous mem- 
brane of the soft palate, of the septum of the nose, and of the inner surface 
of the lower lip. The appearances at the site of inoculation, the body 
temperature, and the general condition of the animal were noted daily. 
The monkeys were killed at various intervals after the inoculation, and 
material for histological study was preserved in Zenker’s fluid. 
EXPERIMENTS IN DETAIL. 
No. 81. Adult male M. cynomologus. Inoculated on the left side of nasal | 
septum, on inside of lower lip, and on the soft palate with vaccine virus No. 1. 
At the time of inoculation some of the virus was also blown into the throat. 
Body temperature 39°.9 C. 
