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No. 89. Adult male M. cynomologus. Inoculated in nose, on lip, and on soft 
palate with vaccine virus No. 1. Body temperature 39°.5 C. 
Twenty-four hours after inoculation the lip shows slight reddening of the: 
mucous membrane about the scratch. The other sites are negative. Body tem- 
perature 39.°5 C, 
Forty-eight hours. Nose and palate negative. Lip: 2 small, red, papular eleva- 
tions, 2 millimeters in diameter, are present. About these the mucous mem- 
brane is reddened for a distance of 1 centimeter. Body temperature 39° C, 
Three days. Nose: A small crust is visible at the site of inoculation. Lip: 
An irregular, opaque, white area, from 1 to 3 millimeters broad and 8 millimeters 
long, surrounded by a red flush, is present. Palate: 2 flat-topped, white eleva- 
tions, 2 millimeters across and 1 millimeter high, seated on a slightly reddened 
mucosa, mark the site of the inoculation. Body temperature 39°.2 C. 
Four days. Nose: Septum swollen and beset with yellow crusts. Lip: An 
irregular elevation from 2 to 4 millimeters wide and 12 millimeters long, dirty- 
white in color, and presenting superficial losses of substance. Lesion sharply 
circumscribed and surrounded by a deep-red areola. Palate: Lesions have in- 
creased in size. Body temperature 39°.6 C. Animal chloroformed. At autopsy 
no specific lesions found other than those at the site of the inoculations. 
No. 90. Adult male M. cynomologus. Inoculated in nose, on lip, and on soft 
palate with vaceine virus No. 1, Body temperature 40° ©. — 
Twenty-six hours ofter the inoculation the animal was chloroformed., No 
reaction was visible at the site of inoculation. No visceral lesions were found at 
autopsy. 
SUMMARY. 
(1) Macroscopic appearance of specific lesions —Lip: After 48 hours 
there appears a small, white or reddish papule, or simply a red area at 
the site of inoculation. 
After 72 hours the mucous membrane is slightly elevated and opaque 
for a distance of 2 millimeters on each side of the scratch. There is a 
faint peripheral flush or areola. . 
After 4 days the opaque area has spread 1 or 2 millimeters, the areola 
is more marked, and the edge of the lesion is more or less sharply cireum- 
scribed. There may be some erosion of the opaque area and it always has 
a macerated appearance. From this time on the lesion spreads slowly, 
presents a rather sharp, elevated, white, opaque border, with a peripheral 
flush, the central portion being more or less eroded. ‘There is no mac- 
roscopic evidence of vesicle formation or of crusting. 
On the palate the lesion runs an identical course and begins to heal 
about the ninth day. 
In the nose, the macroscopic appearances are unsatisfactory, owing to 
the swelling of the mucous membrane which prevents close inspection. 
When the inoculation is at all near the anterior nares, there is a decided 
tendency for the lesion to spread out over the skin about the nostril, where 
it takes on the characteristics of a skin inoculation. 
(2) Constitutional reaction—No general constitutional reaction was 
observed. The temperature reaction was not marked, although there was 
