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constant presence of the cytoplasmic phases of cytoryctes variole in the 
epithelial cells of the lesion and the absence of nuclear phases is consistent 
with the hypothesis that the latter are peculiar to variola, for as we will 
show later, the epithelial cells of the mucous membrane present many 
examples of the nuclear phases of the organism when variola virus is 
used for inoculation. 
CONCLUSIONS. 
(1) Vaccination of the monkey, M. cynomologus, upon the nasal, oral, 
or buccal mucous membrane gives rise to a true vaccine lesion similar to 
that which follows vaccination of the skin. 
(2) The vaccine lesion on the mucous membrane shows certain dif- 
ferences from that upon the skin, but these differences are explained by 
the physical conditions at the locus of inoculation. 
(3) The presence of protoplasmic phases of cytoryctes variole and 
the absence of nuclear phases of the organism in the vaccine lesion on 
the mucous membrane is consistent with the hypothesis that the former 
cycle is associated with the lesions of vaccinia and that the latter do not 
occur in such lesions, being peculiar to variola. 
po Eee a Se or 
