PART II. 
STUDIES UPON EXPERIMENTAL VARIOLA IN MONKEYS (MA- 
CACUS CYNOMOLOGUS AND M. NEMESTRINUS) AND IN 
THE ORANG-UTAN (SIMIA SATYRUS). 
1. VARIOLA INOCULATA FOLLOWING INOCULATION OF THE SKIN OF THE 
MONKEY. 
In these experiments particular attention was paid to the evolution of 
the specific lesion at the site of the inoculation, to the general exanthem, 
and to the constitutional reaction of the animal. Material was collected 
for the histological study of the primary lesion, of the exanthem, and of 
the internal organs. 
Technique-—The different strains of virus used were obtained from 
cases of smallpox occurring among the European and native population 
of Manila and of the provinces. So far as circumstances permitted, the 
virus was kept on ice from the time of collection until used for inocula- 
tion. . 
In some experiments the contents of the pustule or the dry disk was 
used, but as a rule the contents of the unclouded vesicle was selected for 
inoculation. Each strain of virus, except one, was inoculated at least 
once upon the skin of a fresh monkey to test its potency. The different 
strains used were as follows: 
No. 21. Collected April 6, 1904, from a case of severe variola vera in a native 
male, aged about 20 years, on the twelfth day of the disease. 
No. 52. Collected May 4 from a case of severe variola vera in an American 
negro in Bilibid Prison, aged about 30 years, on the tenth day of the disease. 
No. 167. Collected from a case of severe variola vera in a native male, aged 
about 20 years. The contents of the unclouded vesicle was collected on the eighth 
day of the disease, August 6, and again on the following day. The contents of 
the pustules was collected on the fifteenth day and the disks from the palms and 
soles on the nineteenth day of the disease. 
No. 199. Collected on September 18 from a case of severe variola vera in an 
American infant on the ninth day of the disease. 
No. 200. Collected on September 17 from a case of severe variola vera in a 
Spanish-Filipino infant on about the ninth day of the disease. 
No. 252. Collected October 7 from a case severe variola vera in a native 
infant, at autopsy, on about the tenth day of the disease. 
No. 307. Collected on October 23 from a case of severe variola vera in an adult 
native male on about the ninth day of the disease. 
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