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Eosinophile cells having round, or slightly oval, granules were also present. The 
cells with the long granules differed from the usual eosinophile cells in having 
a more distinct cell membrane than the regular type of eosinophile. In this 
respect they conformed more closely to the type of the polymorphonuclear 
leucocyte. 
Bone marrow and testicle: As these organs present the only specific visceral 
lesions of smallpox in man they were scrutinized with care in our variolated 
monkeys. No macroscopic evidence of focal lesions in these organs were seen at 
autopsy, and the bone marrow from four cases and the testicle from twelve were 
negative in this respect when studied microscopically. 
Liver, spleen, kidney, and lung: No pathological process was demonstrable either 
macroscopically or microscopically in these organs. 
SUMMARY. 
1. (a) The macroscopic appearance of the primary lesion in Macacus 
cynomologus.—Twenty-four hours after inoculation there is some eleva- 
tion and some opacity of the skin about the scratch. The process has 
rarely a lateral extent of more than 2 millimeters. 
After 48 hours the appearance is usually the same. 
After 3 days the elevation is more marked and the opacity gives place 
to a pink or red appearance. So far as can be discovered by the naked 
eye, the specific process may now be said to have begun. Previous to 
this the reaction has been in no way different from that which follows a 
simple scratch, without the introduction of virus. 
After 4 days the lesion usually attains a breadth of 6 or 7 millimeters 
and is present as a distinctly pink or red elevation, which is firm to the 
touch and bears on its summit a narrow crust which has its origin in the 
drying of the serum exuding at the time of inoculation. However, the 
crust has somewhat increased in extent. At this time the skin about the 
crust is more or less translucent. It is often impossible to say whether 
or not this translucence is, at this time, the site of a definite vesicle. 
After 5 days the lesions show the characteristics of a pock, being re- 
solvable into rather distinct zones which correspond with those dis- 
tinguishable in the microscopic sections. Going from the center of the 
lesion toward the periphery, we recognize in turn—first a crust, second a 
vesicular ring, third a zone of elevation and hyperemia. The.lesion now 
has a lateral extent of from 6 to 8 millimeters. The whole lesion is 
more or less elevated upon a broad indurated base, due to cedema of the 
skin and subcutaneous tissues. 
After 6 days the picture is the same, save that each of the zones has 
extended peripherally. At about this time the profile of the lesion 
undergoes a change in that it loses its flowing outline as a more or less 
hemispherical elevation, and takes on a flat-topped, plateau-like appear- 
ance. In going from the center outward we pass along the fairly flat 
crust, then over the vesicular ring, which attains a greater elevation than 
the crust, forming a rampart, and then come to an abrupt declivity where 
the zone of hyperemia or areola extends into normal skin. 
