7 oa -. ’ Pa Sl pe le red lr ek | i il “< VE, ee ee 
291 
of the greatest swelling the nodes are markedly tender. After the ninth 
or tenth day the nodes become smaller but remain firmer than normally 
for a considerable length of time. 
The viscera of the animals killed during the disease showed no macro- 
scopic lesions. Particular attention was paid to the bone marrow and 
testes, as these organs are the site of the only specific visceral lesion in 
variola vera in man. 
(c) Inoculations of the skin with variola disks—The inoculations in 
this series of animals resulted in a primary lesion which conformed to 
the type described above, save that the whole process was retarded in its 
evolution. However, the occurrence of the exanthem is in sharp contrast 
to that on the animals inoculated with the contents of the variola vesicle. 
The exanthem occurred in only 3 of the 5 animals, appearing in one on 
the eighth day, in the other 2 on the ninth day of the disease. In these 
3 animals a total of only 7 eruptive lesions were found. The exanthem 
passed through its evolution rapidly and the individual lesions were small. 
‘The temperature reaction was like that in the preceding experiments in 
3 of the animals, while in 2 it was indefinite. In other respects the 
results were similar to those in which the contents of the variola vesicle 
was used for inoculation. 
(d) Inoculation of the skin with dried pustule.contents—In both 
animals of this series the primary lesion was typical in its development 
but somewhat delayed in its evolution. No exanthem was observed. The 
temperature reaction was the same as in the animals inoculated with the 
contents of the variola vesicle. 
(e) Inoculation of Macacus nemestrinus.—In this monkey there is 
considerable variation from the type of the disease seen in Macacus 
cynomologus. This difference is principally shown in the evolution of 
the primary lesion. In this species the vesiculation of the lesion is 
much less definite, and there is an exaggeration of the cedema beneath it. 
The exanthem differed in no way from that observed in the Philippine 
monkey. ‘The temperature reaction was less definite. In other respects 
the results of the inoculations were similar. 
2. (a) Histological examination of the primary lesions.—The evolution 
of the lesion at the site of inoculation is characterized by a combination 
of degenerative, exudative, and reparative processes. Some one of these 
phenomena dominate the picture at different stages of the lesion, and in 
these various cell types figure. ; 
The earlier lesions collected during the first, second, and third days of 
the disease primarily present the process of repair of a simple wound 
of the skin, but to this is added a change in the epithelial cells which 
border the incision. In these cells some degree of swelling and of 
degeneration is evident, although the usual reparative power of the rete 
is retained in sufficient degree to bridge the defect caused by the inocula- 
tion during the third day of the experiment. The cytoplasmic phases 
39714 —6 
