301 
cutaneous lesions of variola inoculata in the monkey in respect to richness 
in forms of Cytoryctes variole, and particularly in the number of nuclear 
forms present. 
3. VARIOLOUS KERATITIS IN MACACUS CYNOMOLOGUS. 
Introduction.—In this section will be considered the results of a series 
of inoculations of the cornea of the Philippine monkey with variola virus. 
These experiments were undertaken to determine what variations in the 
type of disease might result from a change in the locus of inoculation ; 
they also yielded material for the histological examination of the specific 
lesion in tissue which is ideal for the study of cell changes owing to per- 
fect preservation and the relative simplicity of the tissue elements. 
T'echnique.—The methods of inoculation and of observation were the 
same as those described in the section upon vaccinal keratitis in the 
monkey. 
Details of experiments.—Highteen animals were employed in this 
series, of which the following experiments are selected to be given in 
detail : 
No, 223. Monkey inoculated on both corneas with virus No. 200. After 48 hours 
slight unevenness was apparent along the line of inoculation. After 96 hours 
a minute defect in the corneal epithelium was present in the inoculated area. 
Chloroformed after 8 days. The cornea presents a small defect in the epithe- 
lium with very slight unevenness about it. No photophobia was observed. There 
was no general exanthem. 
Histological examination showed proliferation of the epithelium about the inoc- 
ulated wound and the presence of cytoryctes. 
No. 224. Monkey was inoculated on the cornea with virus No. 200. After 48 
hours the cornea was slightly uneven along the line of inoculation. Photophobia 
and haziness of the cornea was observed after 96 hours. Conjunctivitis was not 
present. On the sixth day of the disease a general exanthem consisting of 5 
smal] vesicles was observed on the face and extremities. On the next day 5 new 
vesicles appeared. The animal was choloroformed on the seventh day. At 
autopsy no evidence of an initial lesion other than that on the cornea was 
demonstrable. 
Histological examination. At the site of inoculation enormous numbers of 
cytoryctes were present in the epithelial cells. No leucocytes were found in the 
lesion or in the corneal substance about it. 
SUMMARY. 
The inoculation of the cornea with vesicle contents was followed by 
the development of a lesion which had much in common with that which 
follows a similar inoculation on the rabbit. The following summary of 
the macroscopic appearances is based upon the observation of 18 experi- 
ments similar to those given above. 
After 24 hours there is some roughening of the surface along the line 
of inoculation. After 48 hours there follows more or less loss of sub- 
stance at the site of the inoculation incision. This loss of substance is 
