304 
(3) The lesion results in less destruction of the corneal epithelium 
than follows similar inoculations of the cornea of the monkey with vac- 
cine virus. 
(4) The lesion on the cornea differs from the variolous lesion on the 
skin of the monkey in that exudation does not play as prominent a part 
and that true vesicle formation does not occur. 
(5) Cytoryctes variole are present in the lesion up to 11 days after 
the inoculation, but nuclear forms of the parasite are not found. 
(6) A variolous lesion on the cornea of the monkey may be followed by 
a general exanthem which appears on the same day as after skin inocula- 
tion. We therefore identify the disease produced in the monkey by 
variolation on the cornea as variola inoculata. 
4. VARIOLA INOCULATA FOLLOWING INOCULATION OF THE MUCOUS MEM- 
BRANE OF THE MONKEY. 
Introduction.—In the preceding sections of this paper we have detailed 
the results which follow the inoculation of the skin and the cornea of 
the monkey with variola virus. We will present here a series of experi- 
ments which show the results of inoculation of that animal upon the 
mucous membrane of the nose, the lip, and the palate. The experiments 
also yielded material for the study of the variola organism and the 
histology of the specific lesion on the mucous membrane. 
Technique-——The method of inoculation and of observation was the 
same as that followed in the series of inoculations of the mucous mem- 
branes of the monkey with vaccine virus. 
Clinical course of the disease—Twenty-nine animals were used in this 
series of inoculations, of which the following are selected to be described 
in detail: 
No. 125. Adult male, Macacus cynomologus. Inoculated on the left side of the 
nasal septum, on the inner side of the lower lip, and on the left palate with variola 
virus No. 167 (vesicle contents). Body temperature 40° C. 
Twenty-four hours after inoculation a slight elevation is noted on the nasal 
septum. The lip and palate are negative. Body temperature 38°.8 C. 
Forty-eight hours. On the lip a narrow white line surrounded by hyperemic 
mucous membrane marks the site of inoculation. Nose and palate negative. 
Body temperature 40° C. 
Three days. The nose shows considerable swelling of the septum, but no dis- 
tinct lesion can be distinguished. The lip presents a white area, 1 X 3 milli- 
meters, which is slightly elevated and has a translucent appearance. The palate 
shows a small, gray spot on the line of inoculation. Body temperature 40° C, 
Four days. Nose negative. The lip shows a white, opaque area 1 X 3 mil- 
limeters, with a ragged elevated edge, about which the mucosa is distinctly red- 
dened. The palate presents a white, slightly elevated area, 2 X 4 millimeters, 
the surface of which is unbroken. Body temperature 40° C, 
Five days. Nose negative. The lip shows an opaque, white area, covered in 
part by the remains of the macerated epithelium, and surrounded by a dull pink 
elevated border. The palate presents an elevated area 2 X 4 millimeters in extent 
and of a gray-white color. About this are smaller similar spots. Body tem- 
perature 39°.5 C, 
