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found scattered over the face, trunk, and extremities. The exanthem consists of 
over 100 lesions. 
Histological examination—Operation wound: Subcutaneous tissue contains 
much fibrin and finely granular precipitate. There is considerable necrosis of 
the underlying muscle. Polymorphonuclear leucocytes in all stages of degenera- 
tion are present in large numbers. Many small blood vessels are thombosed, 
and in them and in the surrounding tissue streptococci are demonstrable. The 
epithelium at the edge of the wound is much degenerated, but no evidence of a 
variolous process can be made out. However, cytoryctes were found in the cells 
of the thickened corium. Trachea: The epithelium is wanting in places and in 
other areas it is somewhat thickened. In many places the basement membrane 
is broken. The epithelial cells show various degenerations. In many groups of 
cells cytoplasmic phases of Cytoryctes variole are demonstrable. The vessels of 
the submucous tissue are much injected, and in one is a mass of fibrin in which 
are phagocytic cells containing streptococci. The tissue is infiltrated with poly- 
nuclear leucocytes. The endothelial cells of a capillary immediately below an 
area in which the epithelium shows cytoryctes are prominent and contain cyto- 
plasmic phases of the parasite. Skin: The lesions of the exanthem present the 
characters which before have been described in similar lesions occurring in the 
course of variola inoculata in the monkey. Mucous membrane; The lesions in 
the cheek pouches consist of areas of degenerated epithelium in which groups of 
cells are stained red, the nuclei having lost their affinity for basic dyes. The 
protoplasm is finely granular, but the cell outlines are retained. Other cells 
are present whose staining properties are unimpaired, and in these are found 
various cytoplasmic phases of Cytoryctes variole. In the upper layers of the 
degenerated epithelium, particularly toward the edge of the lesion, the cells 
are separated by fluid. In some places the appearance is similar to that seen 
near the edge of a 4-day primary lesion in the skin. Polynuclear leucocytes are 
present in the epithelium and in the tissue beneath, although in the latter situa- 
tion the reaction is much less intense than it is beneath a primary lesion of the 
mucous membrane. (sophagus:—Small areas are present in which the epithe- 
lial cells are swollen and their nuclei shrunken. Evidence can be made out of 
accumulation of fluid in and between the cells. Cytoplasmic forms of Cytoryctes 
variole are present in many of the cells. Comparatively few polynuclear leu- 
cocytes are present in the lesion, and there is practically no reaction in the tissue 
beneath. Seminal vesicles: Focal lesions are present in the septa of the tubules, 
The lesions consist of collections of degenerated cells and fibrin which lie in the 
connective tissue stalks of the septa. Associated with this there is more or less 
degeneration of the adjacent epithelial cells. A few polynuclear leucocytes are 
present. Many of the epithelial cells contain cytoplasmic phases of Cytoryctes 
variole. Testicle: Normal. Bone marrow: No focal lesions are demonstrable. 
The lung, liver, spleen, and kidney show no lesions, 
Summary.—From observation of the trachea in animals killed at 
various times after the inoculation it was seen that a distinct process 
occurs at the site of inoculation. Our data on this point seems to us 
insufficient to form the basis of a description of the stages in the evolu- 
tion of the specific lesion in this situation. 
A general exanthem was observed in 3 of the 4 monkeys of this series. 
The single animal which did not show an eruption died on the eighth 
day of the experiment. The exanthem appeared on the seventh day in 
1 animal, on the ninth day in the other 2. The exanthem was very 
extensive and in the 2 animals in which it appeared on the ninth day 
